Effects of Tricholoma matsutake (Agaricomycetes) Extracts on Promoting Proliferation of HaCaT Cells and Accelerating Mice Wound Healing

Tricholoma matsutake is popular in Asian countries because of its edibility and medicinal use. T. a precious natural fungus, it widely used food biological products. This study aimed to explore the mechanism on promoting proliferation human immortalized keratinocyte (HaCaT) cells accelerating wound healing mice. The MTT assay was test effects three different extracts (0, 62.5, 125, 250, 500, 1000 ?g/mL) HaCaT cell viability. were treated with (100, 500 morphological biophysical properties detected by atomic force microscopy JPK data processing. Western blot analysis Notch signaling pathways 50% ethanol extract (50%T) for 24 h ?g/mL). Mouse wounds 50%T 15 days. Wound observed back skin mice at times. quality estimated histological staining (hematoxylin eosin Masson's trichrome). All counted GraphPad Prism 5 software. increased concentration remarkably promoted proliferation. Young's modulus showed biggest increase from 1.73 ± 0.13 kPa (0 4.57 0.16 (500 group. Notch1/Jagged1 upregulated an 100, Moreover, compared negative positive control groups, epidermal regeneration, subepidermal tissue formation, collagen deposition. results that promotes not only but also